Facs Canto

FACSCanto™ Flow Cytometer From BD The BD FACSCanto™ is often hailed as the new generation of flow cytometry One striking difference between the FACSCanto™ and older generation machines such as the FACSCalibur™ is that this system operates on a PCbased Windows XP® platform using BD FACSDiva™ software.

Facs canto. The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easytouse yet powerful sixcolor benchtop analyzer. BD Biosciences proudly announces the launch of the BD FACSCanto™benchtop flow cytometry system This is a dual laser, 6color instrument with powerful software to make multicolor experimentation fast and simple The system’s design creates an instrument of unrivalled technical superiority in a compact and easytouse package. FACSCanto II PMI Kit Includes sheath/cleaning/rinse filters, debubble filter, tubing, air filters, Orings, waste carboy cap SKU.

Flow Cytometry And Cell Sorting Facility. The FACSCanto is a data acquisition flow cytometer that combines a patented optical design, digital electronics and a novel sample injection system supporting carryover of less than 01% Highspeed data processing, industryleading sensitivity, and minimal sampletosample carryover make this instrument uniquely suited for rare event analysis. Thank you for all your answers The problem has been solved Erroe 'e040' of FACS CantoII means that the tube sensor cannot detect if the tube is present or gives a wrong signal that the tube is.

How to operate the BD FACSCanto flow cytometer Preface Dear colleague, the BD FACSCanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day. Optimizing Cytometer Settings BD FACSCanto Clinical Software Before you record data for a sample, optimize the detector voltages, spectral overlap, and threshold settings for the sample type and fluorochrome to be run 1 Follow the prompts on the screen to proceed with optimization • Click Yes to save results. BD FACSCanto II flow cytometers were used in six laboratories and BC Navios flow cytometers in four laboratories All 3laser instruments were equipped with their standard optical configuration for a blue laser emitting at 4 nm, a red laser emitting at 633 nm (BD FACSCanto II) or 638 nm (BC Navios), and a violet laser emitting at 405 nm.

The BD FACSCanto II system is intended for use as an In Vitro Diagnostic device for identification and enumeration of lymphocyte subsets in human cells in suspension for flow cytometry. BD FACSCanto™ II Flow Cytometer Filter Guide 1For Research Use Only Not for use in diagnostic or therapeutic procedures 2For 6color immunophenotyping only Seven and eightcolor immunophenotyping on this device require validation by the user Class 1 Laser Product. The BD FACSCanto™ II system features many innovations At the heart of the cytometer, for example, the fluidics system features a fixedalignment flow cell to minimize startup time and improve reproducibility The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance.

Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easytouse benchtop analyzer that delivers proven performance, accuracy, and high quality results. Flow cytometer Beckton Dickinson FacsCanto It consists of 7 light sensors, PMT, Laser Blue 4 nm and Red Laser 633 nm It consists of 7 light sensors, PMT, Laser Blue 4 nm and Red Laser 633 nm Preamps for each PMT. FACS Canto II APCCy7 APCH7 APCAlexa750 Department of Immunology Cell Analysis Facility University of Washington Michele Black, Director mblack2@uwashingtonedu FACS Canto II Canto 2 3 lasers Standard Filter & Dye set Pacific Blue BV421 V450 V500 V550* Pacific Orange* Violet Laser 405nm C B A B l a n k B l a n k.

AccessGUDID BD FACSCanto™ () BD FACSCANTO System SW V30 W/Dongle. Cell analyzer with proven reliability The BD FACSCanto™ family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters Numerous innovations make the system easy to use, powerful, and productive. Scheduler FACSCanto (405/4/633) MCRB 621 Contact UFCR, flowsort@umnedu or UFCR Billboard Please look here for important information regarding this instrument.

We have a FACSCanto II with blue, red and violet lasers and every once in a while we will get laser power warnings on either or both of the red and violet lasers Usually we just restart the instrument and things are fine Recently this has become more frequent especially with the violet laser and is requiring multiple restarts before the laser. R5087A FACSCanto's, MACSQuant's R5503 ImageStreamX, Aurora Sorting Rooms R5503 FACSAria's, SH800S R5504 M00 R5529 MoFlo XDP Luminex R5503 Analyzer Hours 24 / 7 for those who have been trained by our staff Sorting Hours 10 am to 5 pm MondayFriday (Staff operated) Contact Us General information, help and analyzer training. When performance matters BD has been innovating flow cytometry since the release of the first commercial flow cytometer in 1974 That tradition continues with the new BD FACSCanto™ benchtop flow cytometer.

Intended Use The BD FACSCanto system is intended for use as an In Vitro Diagnostic device for identification and enumeration of lymphocyte subsets in human cells in suspension for flow cytometry System Components The BD FACSCanto system consists of a benchtop flow cytometer, a self contained fluidics cart, and the BD FACSCanto workstation. FACS Canto II Quick User Guide Page 4 11 It is advisable to run a series of 3 cleans of 5 minutes each before starting The tubes are in the rack at the side of the FACS Canto and run them in the order of Bleach, FACS Rinse and Deionised water Top up the tubes from the wash bottles if required. BD Influx sorter (formerly in the RAb Lab facility) BSL1 only Staff use only, inquire 3 lasers 350nm UV with 2 detectors, 4nm with 5 detectors,633nm with 2 detectors.

BD FACSCanto II BD FACSCanto II Book Now To request a service, sign into CrossLab This instrument incorporates blue, red and violet lasers and can collect eight colors of fluorescence from the blue to far red areas of the spectrum This instrument runs FACS Diva software. The BD FACSCanto™ II system features many innovations At the heart of the cytometer, for example, the fluidics system features a fixedalignment flow cell to minimize startup time and improve reproducibility The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance. BD FACSCanto IITM 11 Subsystems Fluidics To introduce and focus the cells for interrogation Optics To generate and collect the light signals Electronics To convert the optical signals to proportional digital signals, process the signals, and communicate with the computer 12 sheath sheath sample Sample Flow.

Scheduler FACSCanto (405/4/633) MCRB 621 Contact UFCR, flowsort@umnedu or UFCR Billboard Please look here for important information regarding this instrument. BD FACSCanto™ II Flow Cytometer Filter Guide 1For Research Use Only Not for use in diagnostic or therapeutic procedures 2For 6color immunophenotyping only Seven and eightcolor immunophenotyping on this device require validation by the user Class 1 Laser Product. When performance matters BD has been innovating flow cytometry since the release of the first commercial flow cytometer in 1974 That tradition continues with the new BD FACSCanto™ benchtop flow cytometer.

Cell analyzer with proven reliability The BD FACSCanto™ family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters Numerous innovations make the system easy to use, powerful, and productive. Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easytouse benchtop analyzer that delivers proven performance, accuracy, and high quality results. FACSCanto The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory provides researchers with training on and access to a FACSCanto ™ analyzer.

The BD FACSCanto II instrument uses lowpowered aircooled and solid state lasers that do not have special power and cooling requirements Page 21 Collection Optics From the flow cell, laser light is routed to the collection optics, which efficiently gather the signals emitted and scattered from each particle The 42 configuration collection optics include two detector arrays, which consist of photomultiplier tubes (PMTs) arranged in one octagon and one trigon as shown in Figure 14. FACSCanto II PMI Kit Includes sheath/cleaning/rinse filters, debubble filter, tubing, air filters, Orings, waste carboy cap SKU. The BD Bioscience FACSCanto II Flow Cytometer is a benchtop analyzer providing reliable and accurate results for experiments with up to 10 parameters The FacsCanto II offers unbeatable consistency and optimal signal detection with increased resolution and sensitivity for multicolor assays.

BD Biosciences proudly announces the launch of the BD FACSCanto™benchtop flow cytometry system This is a dual laser, 6color instrument with powerful software to make multicolor experimentation fast and simple The system’s design creates an instrument of unrivalled technical superiority in a compact and easytouse package. A proven platform for high reliability and quality results Built on more than 30 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ flow cytometry system delivers reliable performance, accuracy, and easeofuse for today's busy clinical laboratories. DESCRIPTION Flow cytometer with 2laser, 6color (42) configuration Uses blue (4 nm) and red (633 nm) lasers to excite fluorescent objects in the flow cell FACSDiva software provides quantification of cell populations, based on fluorescence emission and forward (FSC) and side (SSC) scattering, related to cell size and shape (granularity).

*BD FACSCanto™ flow cytometry system Solid State 4 Green FITC Alexa Fluor® 4 Yellow PE Red PerCP PerCPCy55 Infrared PECy7 HeNe 633 Red APC Alexa Fluor® 647 Infrared BD APCH7 APCCy7 *†BD FACSCanto™ II flow cytometry system Solid State 4 Green FITC Alexa Fluor® 4 Yellow PE Orange PETexas Red®b Red PerCP PerCPCy55. BD FACSCanto II Operation Manual 1 FACSCanto II Standard Operation Protocol Basic Operation 1 Make sure the following steps are done before running your samples a Waste tank is empty and refill the bottom layer of waste tank with bleach b There is enough amount of sheath fluid to perform machine start up and sample acquisition 2. The BD FACSCanto™ family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters* Numerous innovations make the system easy to use, powerful, and productive A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility.

BD Biosciences proudly announces the launch of the BD FACSCanto™benchtop flow cytometry system This is a dual laser, 6color instrument with powerful software to make multicolor experimentation fast and simple The system’s design creates an instrument of unrivalled technical superiority in a compact and easytouse package. The FACS Canto II is an easy to use, powerful, and productive instrument that allows the counting of cells smaller than 5 µm in a water sample It is equipped with 3 lasers (blue 4 nm, red 633 nm and violet 405 nm) that impact with each individual cell and separate them according to their fluorescent properties. BD FACS Canto and associated Fluidics Cart Product Usage Flow cytometer for use in In vitro Diagnostics Code Information.

BD FACSCanto/BD FACSCanto II BD LSR II tightening nut If the coupler continues to leak after you tighten the fitting, remove and then reinstall the coupler as follows Page 110 See Replacing a Pump Syringe on page 125 • BD LSR II and BD FACSCanto Absorbent pad Inspect the absorbent pad at the back of the HTS unit to make sure it is not. This video describes how to set up an experiment in FACS Diva version 80 on an LSR II flow cytometer. BD Biosciences FACSCanto Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easytouse benchtop analyzer that delivers proven performance, accuracy, and highquality results.

Are acquired on a BD FACSCanto II flow cytometer and analyzed with BD FACSCanto clinical software with BD Stem Cell Enumeration module v10 The software uses an automatic gating algorithm to identify and isolate stem cells Software acquisition and analysis methods are based on the method featured in the Clinical and Laboratory. § BD FACSCanto™ II BD LSRFortessa™ X BD FACSAria™ Product Family BD Infl ux™ BD FACSJazz™ BD Horizon™ BUV395 Violet 348 355 395 † Hoechst Blue 350 355, 375 461 ††† BD Horizon™ BV421 Blue 407 405 421 †††††† BD Horizon™ V450 Blue 404 405 448 †††††† Pacifi c Blue™ Blue 401 405 452. The BD FACSCanto II system is intended fo r use as an In Vitro Diagnostic device for identification and enumeration of lymphocyte subsets in human cells in suspension for flow cytometry Chapter 1 Introduction15.

BD FACSCanto II system features many innovations The optics of the BD FACSCanto II system consist of an excitation source with up to three lasers blue (4nm, aircooled, mW solid state) & red (633nm, 17mW HeNe) A third violet (405nm, 30mW solid state) laser can be added at an additional cost. Most of the interactions that a user has with a flow cytometer is with the fluidics system, and many of the issues that users will face in troubleshooting problems on the instrument will also be here Understanding how the fluidics system works on your flow cytometer will help you prevent many common issues, prepare your samples correctly, and protect your data Here are four important things. FACSCanto II BSL1 samples only, no unfixed human cells 3 lasers 4nm with 4 detectors, 405nm with 2 detectors, 633nm with 2 detectors optical configuration;.